Título: Luminescent monitoring of kanamycin based on the joint use of split aptamers and silver nanoclusters
Resumen: The analytical potential of synthesizing metal nanoclusters (NCs) has been explored using oligonucleotides as interaction ligands. These can aid in developing rapid-response luminescent systems to assess their potential as analytical tools for determining compounds that modify their spectroscopic characteristics. Moreover, their combined application with split aptamers has introduced a novel research approach that is beneficial in developing biosensors for xenobiotic compounds in aquaculture. In this research, a fluorescent tunable biosensor was created by linking a pair of nanocluster beacons (NCBs), consisting of DNA-silver nanoclusters (DNA-AgNCs) and a G-rich enhancer sequence (GRS), to the ends of both split aptamers. The NCB enhanced fluorescence of the DNA-AgNCs due to their proximity to the GRS, which occurs when both split aptamers bind in the presence of kanamycin. The study has optimized the physicochemical and instrumental variables influencing the luminescent OLG-AgNCs complex formation performance and the aptameric reaction with kanamycin. The method allows determining kanamycin with a limit of detection of 12.52 nmol L-1 in a linear range of 21.66 – 5·103 nmol L-1. By comparing the use of split aptamers to the complete aptamer and other procedures, the study has demonstrated the superiority of split aptamers and GRS concerning analytical features and incubation time. Application of the biosensor to water samples from aquaculture facilities and wastewater treatment plants, with recoveries of 87.29 to 102.38 %, confirmed its specificity and reliability, highlighting its potential for antibiotic monitoring in the industry.
Revista: Microchimica Acta
