Título: IN VIVO STUDY OF THE ANTI-INFLAMMATORY AND INMUNOMODULATORY EFFECTS OF ULVA RIGIDA-DERIVED POLYSACCHARIDES ON TISSUE REGENERATION IN DANIO RERIO
Resumen: The search for new pro-regenerative drugs focuses on substances of a variety of origins with biological activities that include immunomodulation, pro-angiogenesis, antioxidation, or anti-inflammation 1,2. In this field, Danio rerio has become a well-established experimental model for drug evaluation due to its genetic, reproductive and developmental advantages.
In this communication, we investigate the anti-inflammatory and immunomodulatory effects of polysaccharides extracted from Ulva rigida (Ulvans) during Danio rerio fin regeneration. Our aim is to assess the regulative potential of these extracts over wound healing, blastema formation and regeneration outgrowth to evaluate potential applications in aquaculture.
These effects were studied over three-month zebrafish adults previously fed for two months with Ulvan-containing diets under a constant feeding regime. At 70dpf, the caudal fins of experimental and control zebrafish were amputated to assess regeneration of this organ over a 30-day period. During this procedure, stocking density, size heterogeneity, and food particle size were controlled. Body length, weight and fin anatomy was measured during this process and morphometrical results adjusted to mathematical growth models. In vivo fluorescence probes for oxidative, mitochondrial or endoplasmic reticulum stress were also studied under confocal and fluorescence microscopies.
Preliminary results suggest a slight amelioration of the morphometrical parameters of fin regenerates of fishes fed with the experimental feeds. This study is part of a global comparison of the biological effects of functional feeds over zebrafish and other fish species of economic interest. This study has been funded by a NextGenerationEU grant, European Union (PCM-00035, Marine Sciences).
Congreso: Aquaculture Europe, Valencia 2025
